Been a very tiring day (last night’s party) and didn’t really work for the whole day. Meeting here and meeting there. Politics and gossips and so on. Enhancement and bug talk. You name it. Lunch time rushed down to “New Men” and collected my pair of trousers. Japanese for dinner. What else? Brought a pair of sport shoes and I am ready to go for my holiday. One more thing, finally had my haircut appointment. Still no news from JP. Probably she has forgotten me. Wanted to dial in Lotus Notes but the server in Paris does not respond.
Check this out. The Nuclear Transfer Technology.
The technique involves several steps. First, the donor cells are grown under special conditions in culture. In this way the number of cells can be increased by several orders of magnitude. It is also possible to make genetic modifications and to select just those cells in which the desired modification has occurred and multiply these up. These cells are then fused with an unfertilized egg from which the introduced nucleus can lead to the formation of an embryo. The embryos are then transplanted into sheep and lambs are born naturally.
- Cells of donor embryo are separated
- Chromosomes are removed from the unfertilized egg
- Donor cell is place next to oocyte and fused it with an electric current
- Embryo develops as though a newly fertilized egg
Check this out as well. The Transgene Technology
Transgenic technology is the introduction of a gene(s) from one species into the DNA of another. In the current methods for production of transgenic animals, the gene of interest is introduced into a fertilised egg and becomes integrated into the host DNA. The gene is linked to a piece of DNA, called the promoter, which makes it active only in certain parts of the transgenic animal. In PPL Therapeutics’ case, genes encoding human proteins are expressed so that the protein is produced in the animal’s milk. Because the protein is produced only in the milk, the animal is normal and healthy.